RNA sequencing provides insight into the mechanism of kinase inhibitor resistance
Alternative drug target identification based on altered gene expression profiles
The parental AN3 CA cell line and the erdafitinib resistant clone 05 were profiled with RNA sequencing, followed by analysis on differentially expressed genes. Image left: Gene set enrichment analysis indicated Myc target genes were upregulated in the resistant clone. Image right: the enrichment plot for Myc target gene set v1.
c-Myc expression is altered in the resistant cell lines compared to the parental cell line. c-Myc protein expression of parental and resistant cell lines were analysed by immunoblotting upon a time-course of erdafitinib treatment.