Mechanistic Cell Biology

Good Understanding of the Mechanism of Action of Your Drug Candidate is Essential to Move it to the Clinic.

Oncolines can help you solve complex questions around drug targeting and compound efficacy. We have broad skills and capabilities in in vitro biology including, immunoblotting (Western blot), flow cytometry, and quantitative PCR. We apply these technologies to determine the inhibition or activation of genes and proteins, to perform pathway analysis, or to reveal the mechanism of action of your small molecule or therapeutic antibody. With these results, the optimal dosage and exposure time of your novel therapeutic entity can be determined. To study the effect of compounds in specific cell models, we can generate cell lines in which your target of interest is modified by gene transfection or RNA interference.

Our scientists have hands-on experience with several functional cell-based assays. Besides our standard proliferation assays with ATP readout, we offer colony-forming unit (CFU) assays, reporter gene assays and ELISA/multiplex assays. If the technique or readout you are interested in is not mentioned on one of our pages, please do not hesitate to contact us.


  • Quantitative polymerase chain reaction (qPCR)
  • RNA sequencing
  • Immunoblotting (Western blot)
  • Flow cytometry


  • Transfection of cell lines
  • Generation of resistant cell lines
  • Knock-down cell lines


  • Reporter gene assays
  • Signal transduction
  • Colony-forming unit assays and 3D culture
  • Co-culture of cancer and immune cells