Synergy testing using dose-response curves of both compounds
Efficient way to test multiple combinations
Measurements in quadruplicate
Bliss Independence Scores are calculated for each condition
Visualization of synergy via gradient in matrices.
The combination matrix shows the best effect of your lead compound in combination with another compound. Your compound will be diluted in a six-points dose range and tested against six doses of the reference compound. In addition, the single dose-response curve of each inhibitor is included in the matrix. The inhibition in proliferation will be determined for each concentration and will be compared to the inhibition observed when compounds are tested as single agents. Differences in inhibition between combination and single agent are calculated as the Bliss Independence Score. A score higher than 0 indicates a synergistic effect. Bliss Independence Scores will be reported for each concentration in the matrix. The sum of Bliss scores and highest Bliss score are reported per matrix.
For more information about the Combination Matrix, please contact us