Oncolines® are proliferation assays with a panel of 102 cancer cell lines covering a diverse set of tissue origins. All cell lines have been genetically characterized for mutations and gene expression, and stocks of low passages are used in the screens to prevent genetic drifting. The quality features result in highly reproducible data. The doubling time of each cell line is monitored in every study. As controls, we always include untreated wells in the studies, as well as the reference compound doxorubicin. Bi-phasic behaviour is recognised by visual inspection of the curves. We do not extrapolate dose response curves for the determination of IC50 values. In case the potency of your compound falls outside the measured range, we will retest the compound in an adapted concentration range to accurately determine the growth parameters.