Pharmacological models to assess the role of tryptophan-metabolizing enzymes in cancer
Cell-based and co-culture assays, tumor mouse models, and screening in primary patient material
Co-culture assay of IDO1-overexpressing HEK-293 cells with lymphocytes from a healthy donor. IDO1 inhibitor NTRC 3883-0 restores proliferation of the fluorescently labeled CD8-positive T cells (left), as indicated by the multiple cell generations present in the T cell population (right).
Illustration: Expression and modulation of IDO1 and TDO in cell cultures isolated from ovarian cancer ascites. The left chart shows a tukey boxplot of basal IDO1 and TDO2 gene expression as determined by qPCR in early passage cell cultures. The right chart shows inhibition of the Trp-catabolizing activity in IFNγ-stimulated samples by NTRC 3883-0 (closed symbols) and epacadostat (open symbols).