Let’s say we need 50 uL of the mixture, IC50 of compound A is 1 nM and IC50 of compound B is 10 nM. In that case we take 25 uL of compound A and 25 uL of compound B. The total of 50 mL is subsequently diluted to generate the dose response curve. Since the compounds are equally effective, 25 uL of compound A can be added to 25 uL of compound B without a ‘dilution effect’.