It is recommended to choose a range that includes the biochemical potency (IC50) of the compound at the lower part of the test concentration range. For example, for a compound with an IC50 of 3 nM, we would advise to use a range of 1 -100 nM.
It is recommended to choose a range that includes the biochemical potency (IC50) of the compound at the lower part of the test concentration range. For example, for a compound with an IC50 of 3 nM, we would advise to use a range of 1 -100 nM.