Flow Cytometry

  • Flow cytometry is a laser-based technology that measures properties of cells flowing in a stream of fluid through a beam of light. Flow cytometry is used to analyze the cell shape, size and properties, and to determine cell numbers and percentages of cells positive for a certain marker.

  • With this technique, we determine protein expression on the surface of cell lines or intracellularly

  • Internalization of antibody-drug-conjugates (ADC) or receptor-ligand complexes can be followed over time

  • We validate knockdown of proteins on the cell surface by means of this technique

  • We determine cell viability (e.g., propidium iodide/Annexin-V) and proliferation (carboxyfluorescein succinimidyl ester [CFSE]) in the absence or presence of compound

  • We design panels to detect and compare marker expression in healthy and/or patient-derived cells

Oncolines® - Cell Line Generation - Transaction of cell lines