Flow cytometry
Flow cytometry is a laser-based technology that measures properties of cells flowing in a stream of fluid through a beam of light. Flow cytometry is used to analyse the cell shape, size and properties, and to determine cell numbers and percentages of cells positive for a certain marker.
With this technique, we determine protein expression on the surface of cell lines or intracellularly.
Internalization of antibody-drug-conjugates (ADC) or receptor-ligand complexes can be followed over time.
We validate knock-down of proteins on the cell surface by means of this technique.
We determine cell viability (e.g. propidium iodide/Annexin-V) and proliferation (carboxyfluorescein succinimidyl ester; CFSE) in the absence or presence of compound.
We design panels to detect and compare marker expression in healthy and/or patient-derived cells.

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