Flow cytometry

  • Flow cytometry is a laser-based technology that measures properties of cells flowing in a stream of fluid through a beam of light. Flow cytometry is used to analyse the cell shape, size and properties, and to determine cell numbers and percentages of cells positive for a certain marker.

  • With this technique, we determine protein expression on the surface of cell lines or intracellularly.

  • Internalization of antibody-drug-conjugates (ADC) or receptor-ligand complexes can be followed over time.

  • We validate knock-down of proteins on the cell surface by means of this technique.

  • We determine cell viability (e.g. propidium iodide/Annexin-V) and proliferation (carboxyfluorescein succinimidyl ester; CFSE) in the absence or presence of compound.

  • We design panels to detect and compare marker expression in healthy and/or patient-derived cells.

Oncolines® - Cell Line Generation - Transaction of cell lines