You may prefer to test a selection of standards of care or experimental therapeutics to combine with your drug candidate. We can assist in selecting mixtures on the basis of biomarkers and targeting, as described by Uitdehaag et al. (2015) – the choice is yours. We are happy to provide cost-free consultation if desired.
The curve shift analysis determines synergy as a decrease in dose to achieve the same effect (dose-based). The Bliss approach bases synergy on an increase in the maximum effect (effect-based). The two methods have been merged into one approach, so that a comprehensive view on synergistic effects is generated. An advantage of the approach is that data interpretation is based on full dose response curves of the single agents and the mixtures. This approach takes the sigmoidal nature of the curves into account, covering exponential effects that may be overlooked in a dose-matrix approach.