The criteria for selection of oncogenes are a.o.:
- Single nucleotide polymorphisms removed
- Only mutations in protein coding sequences retained
- Mutations filtered for occurrence in cancer patients
The mutant status of relevant oncogenes and tumor suppressors in the cell lines, e.g. B-RAF, CDKN2A, CTNNB1, EGFR, H-RAS, K-RAS, N-RAS, PIK3CA, TP53 etc. was confirmed by in-house DNA sequencing.