The criteria for selection of oncogenes are a.o.:

  1. Single nucleotide polymorphisms removed
  2. Only mutations in protein coding sequences retained
  3. Mutations filtered for occurrence in cancer patients

The mutant status of relevant oncogenes and tumor suppressors in the cell lines, e.g. B-RAF, CDKN2A, CTNNB1, EGFR, H-RAS, K-RAS, N-RAS, PIK3CA, TP53 etc. was confirmed by in-house DNA sequencing.