An advantage of NTRC’s approach is the use of full dose response curves. This takes the sigmoidal nature of drug-effect relations into account, as illustrated on the right (Chou, 2006). The exponential behaviour may be overlooked in a dose-matrix approach, which may lead to a misinterpretation of the combination effects.
In case a synergy is found in a SynergyFinder™ study, an experimental replicate will be performed as part of the workplan. The reproducibility of NTRC’s cancer cell line profiling methods is very high (Uitdehaag et al., 2015; Uitdehaag et al., 2016). NTRC also offers SynergyScreen™, in which one compound is profiled against a large library of reference anti-cancer agents.